ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
P. Patel1, H. Desai2, R. Krishnamurthy1 and A. Shah3
1C. G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Gujarat, India.
2Main Cotton Research Station, NAU, Gujarat, India.
3Prasad Biotech, Gundlav, Valsad, Gujarat, India.
J Pure Appl Microbiol. 2017;11(3):1549-1557 | © The Author(s). 2017
Received: 10/07/2017 | Accepted: 25/08/2017 | Published: 30/09/2017

Among the collection of non-pathogenic endophytes isolated from the flower of mahua, one bacteria (Bacillus amyloliquefaciens) and two yeasts (Rhodotorula sp. and Pichia kudriavzevii) were selected based on their ability to promote growth of greengram. The response of greengram for the growth, yield and nutrient uptake with the microbial inoculation of these three isolates was analyzed individually and in combination under two different field conditions. The experiment was laid out in randomized block design which revealed that single inoculants of both the yeast isolates with biocompost application found superior than recommended dose of chemical fertilizer. Further, the single inoculation of P. kudriavzevii and Rhodotorula sp. application recorded 31.80 % and 26.93 % more economical and 15.02 % and 12.49 % higher stover yield, respectively than sole biocompost application. The yeast isolates, P. kudriavzevii and Rhodotorula sp. also recorded 1.31 % and 0.15 % increase in harvest index compared to chemical fertilizer and 10.30 % and 9.04 % increase than sole biocompost application, respectively. Data showed that combinations of the bacteria and yeast inoculums had no additional effects with respect to their single inoculation, but showed superior performance compared to uninoculated controls. This study indicates that a single microbial formulation significantly increases the plant growth and yield in comparison with dual and triple inoculums. The single inoculam of P. kudriavzevii showed significantly higher results than in comparison with B. amyloliquefaciens and Rhodotorula sp. Therefore P. kudriavzevii can be a good alternative for chemical fertilizer under South Gujarat region especially for greengram cultivation.


Greengram, Bacillus amyloliquefaciens, Rhodotorula sp., Pichia kudriavzevii.

Article Metrics

Article View: 1635

Share This Article

© The Author(s) 2017. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.