ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
K.V. Smitha1, 2 and B.V. Pradeep1
1Department of Microbiology, Karpagam University, (Karpagam Academy of Higher Education), Coimbatore – 641021, Tamil Nadu, India.
2Department of Microbiology, Safi Institute of Advanced Study, Vazhyoor – 673 633, Malappuram, India.
J Pure Appl Microbiol. 2017;11(3):1447-1456
https://doi.org/10.22207/JPAM.11.3.28 | © The Author(s). 2017
Received: 20/06/2017 | Accepted: 25/08/2017 | Published: 30/09/2017
Abstract

A bacterium which produced novel extracellular fibrinolytic enzyme for digesting bovine blood clots was isolated from soil, and identified by 16s rRNA sequencing as Bacillus altitudinis, given strain name was S-CSR 0020 (accession number KT369312). Fibrin proved the best nitrogen source with an enzyme activity of 750 U/mL, followed by casein after incubation at 37 °C for 4 days. The cultural conditions were optimised using Response Surface Methodology (RSM) and Box-Behnken Design (BBD). Based on 3D surface plot and contour plots, the optimized temperature, pH and substrate concentrations were 47°C, 10.5 and 4 g/L respectively, resulted in increase in enzyme activity of 306.88 U/mL and specific activity of 780 U/mg which was 2-fold; compared to initial level of 400 U/mg after 2 days of incubation. The crude enzyme has got potent activity and digested human blood clot completely within 1hr.

Keywords

Bacillus altitudinis S-CSR 0020, bovine blood clot, Box Behnken Design, fibrinolytic enzyme.

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