S.L. Perez Martel1, O. Romero2, I. Trevino3,
G. Delgado4, B. Petlacalco4,F. Romero4, H. Hernandez4,
Valencia De Ita Ma.A2  and V. Rodriguez-Hernandez1*

1Colegio de Posgraduados, Campus Puebla-Mexico.
2Centro de Agroecologia-ICUAP; Benemerita Universidad Autonoma de Puebla-Mexico.
3Facultad de Medicina Veterinaria-BUAP.
4CyTA Labs.


 Nine dairy cows seven months gestation received via the milk ducts formulation inactivated antigen protein adhesion to fibronectin of Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli cells. The analysis of the immunoglobulins G by indirect ELISA against the applied antigens, showed that five of nine cows were stimulated with Escherichia coli, the case for the antigenic stimulus for Streptococcus agalactiae showed that eight of nine cows responded with good indices of significance. Finally, stimulus virulence proteins of Staphylococcus aureus adhesion to fibronectin, revealed response rates all cows (P <0.0002). Intraductal response to antigens promises to be a way for immunization to dairy cows in the drying stage.

Keywords: Acute mastitis, inactivated antigens and analysis of immunoglobulins.