Syed Sadaqat Shah1,2, Yaseen Ur Rehman2, Arshad Iqbal2,
Zia Ur Rahman3, Bangwei Zhou1, Mu Peng4 and Zhijian Li1*

1Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Science, School of Life Science, Northeast Normal University, Changchun 130024, China.
2Department of Botany, Islamia College Peshawar, Peshawar, KPK, Pakistan.
3Department of Environmental Sciences, University of Haripur, Pakistan.
4College of Life Science, Northeast Forestry University, Harbin 150040, China.


The current research was conducted to study the qualitative analysis and antimicrobial activity of different extracts of Viscum.albumL. Screening of Viscum album for their chemicals was led by familiar qualitative procedures, which exposed the existence of a number of bioactive compounds including alkaloids, saponins, tannins, flavonoids, terpenoids, steroids, reducing sugar and phenols. The antimicrobial activities were investigated by disc diffusion method. Investigation of the data discovered that all of the five extracts of Viscum album stems, leaves and fruit showed diverse range of antimicrobial activities. Ethyl acetate, butanol, water and crude extracts showed maximum inhibitory effects against all the microbial species. Especially, Gram-positive bacterial and fungal pathogen. The most liable, gram-positive bacteria were Bacillus subtilis, Bacillus atrophaeusand Staphylococcus aureus, which were repressed by all extracts except n-hexane. The most susceptible gram-negative bacterial species were Escherichia coli, Erwiniacarotovoraand Agrobacterium tumefaciens where as the most resilient gram-negative bacterium was Salmonella typhi. The results have also supported the practice of aqueous extract were found to be in effect against Salmonella typhi and Escherichia coli. Thus, our findings have provided support for the use of Viscum album stems, leaves and fruit in traditional medicines.

Keywords: Phytochemical screening; antimicrobial activities; Viscum album; bacteria; disc diffusion method.