Herlina Rante, Risfah Yulianty, Yayu Mulsiani Evary
and Elvira Hardiana

Pharmacy Faculty of Hasanuddin University, South Sulawesi, Indonesia.

ABSTRACT

This study aims to identify endophytic fungi from medicinal plant Mellochiaumbellataand investigate  their  antibacterial  potencies. Twelve species of endophytic fungi weresuccessfully  isolated  from  Mellochiaumbellataleaves and twigs. Four of them showed an activity in the antagonist test against Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Shigelladysentriae. The active isolates were fermented in potato dextrose yeast (PDY) medium at 25oC for 21 days with agitation of 150 rpm. The secondary metabolites were extracted from the fermentation medium using ethyl acetate and the mycelia were extracted using methanol. The ethylacetate and methanol extracts  were  assessed  for their antibacterial  activity  against the pathogenic  bacteria and some of the isolates extract showed antibaterial activity with inhibition zone obtained were between 0 and 10,51 mm.From ethyl acetate extracts, We were obtained MUD5 94 mg  withinhibition zone 8,08 mm against E. coli, 10,51 against S.dysentriae, 7,81 mm against P. aeruginosa, 9,00 mm against V. cholerae, MUR4 116 mg with inhibition zone 8,61 mm against E. Coli, MUR5 was obtained 93 mg with inhibition zone 7,54 mm against E. coli, and  MUR6 96 mg with inhibition zone 7,44 mg against P.aeruginosa. While from methanol extracts were obtained MUR6 516 mg with inhibition zone 9,3 mm against P. aeruginosa. Phytochemical identification revealed that the active extracts contain alkaloids, flavonoids and steroids. From this present work, it can be concluded that these endiphytic fungi could be promisng source of bioactive compounds and can be used for further study.

Keywords: Antibacterial Activity; Endophytes;MellochiaUmbellata; Mycelia.