Removal of Erionyl blue A-R and Solophenyl Black FR Textile Dyes using Enzymatic Extracts of Laccases of Pleurotus ostreatus and Pleurotus djamor

AIA Alonso-Calderon¹, E. Chavez-Bravo², E. Vidal-Robles³,
S. Martinez-Galindo⁴, J.R. Omaire-Vargas⁴ and M.A. Marin-Castro⁵

¹Chemical Engineering Faculty, Autonomous University of Puebla, Mexico.
University City, Avenue San Claudio and south 18, Colony San Manuel.
²Center for Research in Microbiological Sciences,
Institute of Sciences Autonomous University of Puebla, Mexico.
³Chemical Engineering Faculty, Autonomous University of Puebla, Mexico.
⁴Engineering Faculty, Autonomous University of Puebla, Mexico.
⁵Agricultural Sciences Research Department of the Sciences-Institute,
Autonomous University of Puebla, Mexico.

ABSTRACT

In this research, extracellular fungal extracts containing Pleurotusostreatus and Pleurotusdjamor were used for the removal of erionyl blue A-R (anthraquinone) and solophenyl black FR (azo) textile dyes in artificially contaminated water at 200 ppm. For erionyl blue A-R, removals of up to 93.6 and 42.85% were achieved, and for solophenyl black FR of 27 and 31.14%, using the enzymatic extracts of P. ostreatus and P. djamor respectively. Enzymatic activity values ​​of 888.41 IU and 152.22 IU were reached for the laccases obtained from the submerged culture extract of Pleurotusostreatus and Pleurotusdjamor respectively. The extract obtained from P. ostreatus was partially purified using dialysis and anion exchange chromatography (DEAE), by polyacrylamide gel electrophoresis. A molecular mass of 67 kDa was determined.

Keywords: Pleurotus, dye, laccase, enzyme.