ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access

Sachidevi Puttaswamy1, Byung-Doo Lee1, Ashley Jurgensmeyer1, Anne Baumstummler2, Kathleen Souza3 and Shramik Sengupta1

1Department of Bioengineering, University of Missouri, 1406, E. Rollins Road, 165 AEB, Columbia MO 65211-5200.
2Department of  Predevelopment – Technology – Collaboration, BioMonitoring, Lab Solutions, MilliporeSigma 39 Route Industrielle de la Hardt; 67120 Molsheim, France.
3Department of Virology and Microbiological Sciences, MilliporeSigma 80 Ashby Rd, Bedford, MA 01886.
J Pure Appl Microbiol. 2017;11(3):1219-1237
https://doi.org/10.22207/JPAM.11.3.01 | © The Author(s). 2017
Received: 13/06/2017 | Accepted: 24/08/2017 | Published: 30/09/2017
Abstract

Existing culture-based instruments for detecting/quantifying proliferating bacteria in suspensions (BACTECTM, BacT/AlertTM, RABITTM etc.) do so based on changes observed in the physical/chemical properties of media (O2/CO2 levels, pH etc.) due to bacterial metabolism. Given the limited metabolic-rate of individual bacterium, they have a “threshold-concentration” of ~107-108CFU/ml, and Times to Detection (TTDs) of 12 hours or longer for low initial loads (<100CFU/ml). We recently developed a method that tracks microbial proliferation in suspensions by monitoring the degree of cell polarization of live microorganisms. In the presence of an AC electric field, there occurs a build-up of charge at the microbial membrane, causing them to act like capacitors. As microorganisms multiply, there occurs a corresponding increase in charges stored in the suspension (“bulk-capacitance”), and this increase in bulk-capacitance serves as our “signature” for presence of live microorganisms. In this study, we explain the theory underlying our approach, establish its applicability to a variety of microorganisms, showing that the “Threshold-Concentration” (nT) for detection is ~103-104CFU/ml, and TTDs are a function of the initial-load(n0) and doubling-time(tD) of the microorganism TTD=1.443*tD*ln(nT/n0) and show that the method can be adapted to obtain the “Most Probable Number” (MPN) of coliforms within 6hrs (vs. >24hrs for existing methods).

Keywords

Viable bacteria; MPN; Rapid Detection; Automated Culture Systems; Bacteria detection; Microfluidics.

Article Metrics

Article View: 1782

Share This Article

© The Author(s) 2017. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.