Chandra Kant Singh1, Ichini Sudhir1, Ramesh Chand1*, Vineeta Singh1 and Mamta Sharma2
1Department of Mycology and Plant Pathology, Institute of Agricultural Sciences,
Banaras Hindu University, Varanasi-221005, India.
2International Crops Research Institute for the Semi-Arid tropics, Patancheru, Hyderabad, 502324, India.
The present experiment was undertaken to study the cultural, morphologicaland pathologicalvariation in six isolates of Phytophthoracajani. The isolates were collected from pigeonpea growing areas of eastern Uttar Pradesh. Isolates exhibited considerable variations in cultural characters and growth on potato dextrose agar medium. The universal ITS1 and ITS4 primers showed the difference among P. cajani isolates. There was significance influence of temperature on the growth of isolates, however isolates could not be differentiated based on the temperature.The average growth (1.69 cm2) at 30°C temperature was recorded with all the isolates followed by average growth (1.25 cm2) at35°C temperature. The maximum radial growth (3.6 cm2)was observed at 96 h in isolate PDC015-1. The isolate PDC013-1 and PDC014-3 showed highest 47.0% plant mortality on ICP 7119 genotype. Among the isolates PDC014-3 killed 28.6% plants after 4th day of inoculation.
Keywords: Cajanuscajan, Phytophthora Blight, Temperature, Variability.