Vinay Hajare*, H. Anandkumar and R.S. Rajeshwari

Department of Microbiology, Navodaya Medical, College, Raichur, Karnataka, India.

ABSTRACT

The H.pylori is one of the most common infections in the world. It causes dyspepsia, chronic gastritis and gastric carcinoma. Spread of the infection from person to person is by oral-oral or faeco-oral route. The aim of the study is to determine the rate of isolation of H.pylori infection from different sites like gastric biopsy, gastric aspirate, saliva and faeces.  Patients were selected based on the clinical findings like early satiation, epigastric pain, epigastric burning, bloating, belching and endoscopic findings. Four types of samples- Gastric biopsy, Gastric aspirate, Saliva and  Faeces were collected and all the four samples were subjected to Direct Gram Stain, Rapid Urease Test and Culture. Culture was done by inoculating samples onto Skirrows media with cefsulodin and amphotericin B and incubated at 35ºC for 3-5 days under microaerophilic conditions. Identification of organism was done based on gram stain of the colony, oxidase, catalase and urease test.  Out of 96 clinically suspected cases of gastritis, H.pylori was isolated in 34(35.41%) patients from Gastric biopsy, 18 (18.75%) patients from Gastric aspirate, 8 (8.33%) patients from saliva and 4 (4.16%) patients from faeces. Among the 96 patients selected 59 were male participants and 37 were female participants with age between 20-60 years.  In the present study, we found that H.pylori can be cultured from biopsy, gastric aspirate, saliva and faeces.  There are several invasive and non invasive techniques to diagnose H.pylori, each having its own advantages and disadvantages. Non-invasive tests on saliva and faeces sample can play an important role in the diagnosis of H.pylori infection, but has the disadvantage of low sensitivity, whereas invasive tests like gastric biopsy and gastric aspirate are more sensitive.

Keywords: H. pylori; Gastritis; Gastric biopsy.