Sathyaprabha Govindaraj1 and Senthil Renganathan2

1Department of Microbiology, Marudupandiyar College, Thanjavur, TN, India.
2Department of Bioinformatics, School of Bioscience, Marudupandiyar College, Thanjavur, TN, India.

Abstract

Pleurotus sp is an edible mushroom, which contains essential nutrients and medicinal properties for living thing. Molecular characterization and genome differentiation of pleurotus sp.studiedusing gel electrophoresis, RAPDand PCR techniques.Separated DNAwas amplified using internal transcribed spacerprimers. 5.8s rRNA sequence submitted to Genbank for comparison and to obtain accession number. Differentiation and identification of Pleurotus sp found by similarity searches(Accession number: JQ740170 and JQ740171). Comparative studies have shownthese two Pleurotus sp (spp1 & ssp2)are closely related to species, 95% -100% identical,Pleurotus Citrinopileatus, Pleurotus populinus, Pleurotus ostreatus, Pleurotus abieticola, Pleurotus pulmonarius, Pleurotus eryngii and Pleurotus sapidus. These Pleurotus sp havebeen studiedfor exhibiting pharmacological potential activitiessuch asantioxidant, antitumor, immunomodulatory activity and hypocholesterolemic through sequence similarity,nucleotide composition, evolutionary studies, secondary structure prediction and conserved region.The study extended to identify the significance of species diversity and fungal samples among various applications.Pleurotus (sp) samplewas subjected to identification of bioactive compounds by using GC-MS. In Pleurotusspp1: Pyridine- 3-carboxamide, 4-dimethylamino-N-(2, 4-difluorophenyl), Piperidin-4-carboxylic acid, Aspidofractinine-3-methanol, (2à, 3á, 5à), Indolizine, and 2-(4-methylphenyl)-. Pleurotusspp2: Imidazolidine, 1, 3-dinitro, Phenol, 2-methyl-4-(1, 1, 3, 3-tetramethylbutyl), and Squalene. Enhancement of more entries as well as sequence comparison and virtual screening might help researchers to acknowledge real world problems between cultivatingnutritional, medicinal and poisonous mushroom.

Keywords: edible Mushroom-internal transcribed spacer-metabolites -pharmacological activities-species diversity.