Gaganpreet Kaur*1, Sanjeev Kumar Soni2 and Rupinder Tewari1

1Department of Microbial Biotechnology, South Campus, New Hospital Building,Panjab University, Sector 25, Chandigarh – 160 014, India.
2Department of Microbiology, South Campus, Basic Medical Science (Block I),
Panjab University, Sector 25, Chandigarh – 160 014, India.

ABSTRACT

Microbial sources for phospholipase A1(PLA1) are economic and industry relevant for degumming of oils and also their product lyso-phospholipids have been widely used as emulsifying agent.Numerous PLAs1have been reported, but still the few enzymes have got position in the commercial sector. Due to enormous demand of PLA1 in the industrial sector, the present study was carried out to optimize PLA1 production using cheaperagro-industrial waste like defatted rice bran. For this, defatted rice bran was used in solid state fermentation for the production of PLA1.One-factor at a time approach was used to obtain maximum production of 51.5U/gmwhich is 2.15 foldsmore than un-optimized medium. The optimized medium componentswere (pH 7): defatted rice bran (5gm), glucose (1% w/v), peptone (1% w/v) and olive oil (0.5 % v/v) with moisture content of 1:1.5 and after 48h of incubation at 37°C. This approach will provide the cleaner solution for degumming of oil as compare to the acid degumming and also help to reduce the stress of the environment by utilizing waste. This is the first report where in Bacillus subtilissubsp.inaquosorumwas employed for PLA1 production viasolid state fermentation.

Keywords: Submerged fermentation, Solid-State Fermentation, Optimization, Classical approach, PLA1, Bacillissubtilissubsp.inaquosorum.