This study was investigated the effect  of  Probiotic (L. plantarum) supplementation at 0 , 1×10⁷,1×10⁸,1×10⁹ cfug^-1 diet  on growth performance, feed utilization, survival rate and carcass quality in Bocourti’s catfish (Pangasius bocourti). An average initial weight of fish 132.59 ±1.20 gfish^-1were divided into four treatments, three replication and fed for 8 weeks. The results showed that body weight, weight gain, daily growth rate, specific growth rate, survival rate, feed conversion ratio and feed conversion efficiency were not significant difference in all groups (P>0.05).However, Fish fed dietary supplementation of probitic at 1×10⁷ cfug^-1 diet  had trendy high in body weight , weight gain, daily growth rate  and specific growth rate with  207.16 ± 16.31 g., 74.30 ± 15.13 gfish^-1, 1.33 ± 0.27gday^-1 and  0.79 ± 0.13%day^-1respectively. The biochemical parameters in serum and carcass quality were did not significantly different for any of the diet treatment. Therefore, this study indicated that the optimum level of L. plantarum in diet for bocourti catfish culturing could be used for contains  1×10⁷ cfug^-1 diet.