Dwi Suryanto*, Rani Artha Munthe, Isnaini Nurwahyuni and
Erman Munir

Department of Biology, Faculty of Mathematics and Natural Sciences,
University of Sumatera Utara, Medan -20115, Indonesia.

ABSTRACT

White root rot disease caused by Rigidoporusmicroporus was a detrimental disease of rubber plant. In controlling the disease, synthetic chemicals were applied. This control may cause environmental pollution and health problem. In this study, an alternative to control WWRD using biological control agent of local Trichoderma spp. isolates was examined. Trichoderma spp. were isolated from soil of healthy rubber, sugarcane and tobacco plantation, and of Sibolangit Forest Park.R. microporuswas isolated from infected rubber tree. Sixteen Trichoderma spp. were isolated and subjected to antagonisms assay againtR. microporus. Trichoderma spp. isolatesshowed to have different ability in inhibiting R. microporus growth. Four isolatesi.e. KA03 of rubber, TB03 of sugarcane, TM01 of tobacco plantations, and HU01 of SibolangitForest Parks showed relatively higher percentage of inhibition to R. microporusby 67.2,66.3, 70.2, and 71%, respectively.Examination of inhibition of white root rot disease in rubber stumpin polyethylene plastic bag showed that the four isolates were able to reduce disease intensity and severity of R. microporusafter 60 days of control, but no improvementof stumpperformance was observed.

Keywords:Biological control, Rigidoporusmicroporus, Trichoderma