Abhijit Manna and Chinnaiah Amutha

Department of Animal Behaviour and Physiology, School of Biological Sciences, Madurai Kamaraj University, Madurai – 625 021, India.

Abstract

Utilisation of fungus and bacteria in bioremediation research is a very common phenomenon in recent time, in this effort, we have also tried to find out the same but in a comparative manner. During this experiment, Trametes versicolor and Bacillus subtilis 1133 were taken into the consideration because of the laccase production ability will give additional advantage to them to detoxify more amounts of toxicants than other non-laccase producers. First, we have seen that Trametes versicolor has significant higher laccase production ability than Bacillus subtilis 1133 determined from the peak obtained in the FPLC, latter on in the degradation of BPA (using ESI-MS), decolourization of industrial dyes (using spectrophotometer) Trametes versicolor showed its significant activity than Bacillus subtilis 1133. At the end we have checked the heavy metal (cadmium) removal activity; here also Trametes versicolor manifested more resistance, adsorption, adaptive nature (in terms of recovering viability), absorption and accumulation of cadmium when compared to Bacillus subtilis 1133.

Keywords: Laccase, ESI-MS, FPLC, cadmium, FT-IR.