Ajayta Rialch, Mary Nisha Tigga, Arun A, S. Samanta
and O.K. Raina
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar – 243 122, India.
Echinococcus granulosusrecombinant proteins including two antigen B sub-units EgAgB8/1 and EgAgB8/2 and Echinococcus protoscolex calcium binding protein (EPC1) were expressed in prokaryotic expression vectors. The diagnostic potential of these three recombinant proteins was evaluated in the detection of cystic echinococcosis in goats in IgG-ELISA. The EgAgB8/1 and EgAgB8/2 recombinant proteins reacted fairly with the hydatid infected goats with sensitivity of 66.7% and80.0% and specificity of 71.3% and 73.3%, respectivelywhile EPC1 recombinant protein showed lower sensitivity (60%) but comparablespecificity (72.3%). Cross-reactivity of these three antigens with goat gastro-intestinal strongyle nematodes and Taenia hydatigenaunder field conditionswas studied. Results showed that EgAgB8/1, EgAgB8/2 and EPC1 antigens cross-reacted with mostof the parasites in the goat host.
Keywords: Echinococcus granulosus; goat; Antigen B8 sub-units;EPC1; Diagnosis; IgG-ELISA; Cross-reactivity.