Ravindra Babu Potti and Mohammed Owaisuddin Fahad
Department of Biotechnology, Sreenidhi Institute of Science and Technology,
Yamnampet (v), Ghatkesar (m), Hyderabad -501301, Telangana State.
The objective of this research is to evaluate alternative source for collagen. The common source of collagen is from bovine and pig. There is need to find an alternative source of collagen due to its demand. Broiler Chicken feet waste is one of the alternative sources because of its abundant collagen protein content. On an average each chicken shop generates minimum of 30-40 kg waste every day in Hyderabad. Collagen is a fibrillar protein composing different forms of the connective tissue: bone, cartilage, tendon and skin. Collagen is the major component of the extracellular matrix, and more than 27 genetic isoforms have been identified. Collagens type I, II and III are the most abundant and well investigated for biomedical applications. Chicken feet, rather than being transformed into meal for animal feed, a large quantity of chicken feet could be used to produce collagen, which is valued for its unique functional properties. The purpose of this research project was to extract and characterize collagen from chicken feet. Biochemical composition of chicken feet collagen such as moisture, protein, fat and ash content was 5.85, 29.11, 35.43 and 28.60%, respectively.Acid- solubilized collagen (ASC) was isolated from the chicken feet. Molecular weight of ASC was analysed and confirmed by SDS- PAGE. Secondary structure of ASC was confirmed by FT-IR spectrum.Chicken feet thus appear to be a good alternative source of high-quality collagen.
Keywords: Collagen, Chicken feet, SDS –PAGE, FTIR. Acid Solubilized Collagen.