S.S. Patel*, N.M. Shah, H.C. Chauhan, B.S. Chandel,
M.D. Shrimali, 
A.C. Patel, K.B. Patel, M.A. Patel, B.K Patel, M.G. Patel, J.K. Kala and Manish Rajgor

Department of Veterinary Microbiology, Veterinary College, S.D.A.U., Sardarkrushinagar, India.


Bluetongue (BT) is an infectious, non-contagious disease of domestic and wild ruminants. Bluetongue virus (BTV) causes severe disease in sheep, which is transmitted by insect vector belonging to Culicoides spp. It is particularly a viral disease of sheep, occasionally affecting cattle, buffaloes, goats, camels and other wild ruminants. Out of 377 (364-blood, 5-spleen and 8-pooled Culicoides) samples 110 (29.18%) and 28 (7.42%) were found positive for BTV antigen by s-ELISA and BT-AGID respectively. Species wise incidence by s-ELISA recorded was 48.20 per cent in sheep, 57.14 per cent in goats and 2.60 per cent in cattle however, none of the blood sample found positive from buffalo and camel. Specieswise incidence by BT-AGID recorded was 12.23 per cent in sheep and 15.71 per cent in goats however, none of the blood sample found positive for BTV antigen from cattle, buffalo and camel. Higher incidence seen in goats by both the test. s-ELISA proved to be the most sensitive in detecting BTV antigen than BT-AGID. Considering s-ELISA as the reference test, the relative sensitivity, specificity and overall agreement between both the tests were 25.45 per cent, 100 per cent and 78.24 per cent respectively.

Keywords: Bluetongue virus, antigen, s-ELISA, BT-AGID.