M. F. Elkady1,2, Soha Farag3* and Ahmed M. Haddad3

1Chemical and Petrochemical Engineering Department, Egypt-Japan University of Science and Technology, New Borg El-Arab City, Alexandria, 21934, Egypt. 2Fabrication Technology Department, Advanced Technology and New Materials Researches Institute, City of Scientific Researches and technological applications, New Borg El-Arab City, Alexandria, 21934, Egypt. 3Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technology Applications, Alexandria, Egypt.

Received on 18 September 2016 and accepted on 24 October 2016



Nano-magnetite was successfully immobilized onto the isolated lead resistant bacteria species using co-precipitation technique to synthesize novel nano-magnetic bacterial bio-composite material. This isolated bacteria was identified as Enterobacter sp. B2 under accession number KT213696. Scanning Electron Microscope micrographs implied that the magnetite nano-particles were dispersed onto the surface of bacteria. The magnetic properties of magnetic bio-composite material were determined using Vibrating Sample Magnetometer (VSM). VSM evident that the bio-composite is characterized by its supermagnetic properties with 50.8 emu/g saturation magnetization that facilitate the material handling and separation. The feasibility of the novel synthesized magnetic bio-composite material for lead decontamination was explored against the variation of the processing parameters during the bioremediation process. The results elucidated that the equilibrium of lead bioremediation process achieved 90.5% within 60 minutes with bio-composite dosage of 0.1 g. The increments of both initial lead concentration and solution temperature have negative impact on the bio-remediation process.

Keywords :

 Magnetic immobilized bacteria; Enterobacter sp.; Bio-composite; Lead removal; Bioremediation