Isolation, Identification and Antibiotic Sensitivity Profiling of Methicillin Resistant Staphylococcus aureus from Bovine Milk in Bihar

Mausam1, P K Ray1, A Dey1, S mohanty1, P Kaushik2, Anjay2, Manju Sinha3 and S K Gupta1*

1 ICAR-Research Complex for Eastern Region, PO:BV College, Patna-800014, Bihar, India. 2 Department of Veterinary Public Health, Bihar Veterinary College, Patna-800014, Bihar, India. 3 Institute of Animal Health and Production, Patna-800014, Bihar, India.

Received on 24 August 2016 and accepted on 27 September 2016

 

ABSTRACT

The aim of this study was to know the prevalence of Staphylococcus aureus in bovine milk samples and to evaluate the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) among S. aureus isolates. A total of 150 bovine milk samples were analysed which showed the prevalence of S. aureus in 56.67% of samples. The mecA gene detection by PCR among S. aureus revealed the distribution of MRSA in 29.33% of bovine milk samples. Antibiotic sensitivity pattern reveals that 86.36 and 95.45 percent MRSA isolates were resistant to penicillin and ampicillin respectively, whereas 80.49 and 87.80 percent non-MRSA isolates were found to be resistant to penicillin and ampicillin antibiotics respectively.

Keywords :

Staphylococcus aureus, bovine milk, MRSA, mecA and ABST.