ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
T. Lurthu Reetha1, J. Johnson Rajeswar2, T.J. Harikrishnan3, K. Sugumar4, P. Srinivasan5 and J. John Kirubaharan6
1Tamilnadu Veterinary and Animal Sciences University, Regional Research, Pudukkottai – 622004, India
2Department of Veterinary Microbiology, Veterinary College and Research Institute, Tirunelveli-627001,India
3Directorate of Research, Tamilnadu Veterinary and Animal Sciences University Chennai -600051,India.
4Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal -637002, India
5Veterinary University Training and Research Centre, Nagapattinam, Tamilnadu, India.
6Department of Veterinary Microbiology, Madras Veterinary College, Chennai – 600007, India.
J Pure Appl Microbiol. 2016;10(4):2821-2826
https://doi.org/10.22207/JPAM.10.4.42 | © The Author(s). 2016
Received: 26/05/2016 | Accepted: 11/07/2016 | Published: 31/12/2016
Abstract

The study was carried out at Veterinary University Training and Research Centre, Tiruchirapalli, Tamil Nadu. A total of 48 day old desi chicks obtained from a private hatchery in Namakkal, TamilNadu were maintained under cage system of rearing up to 52 weeks of age as per standard management practices. All the 48 chicks were divided into six groups having eight chicks in each group were subjected to different treatment regimes. Serum samples were collected  at 21 days interval  and the post vaccination antibody titre was assessed by ELISA tests. All the birds were challenged at 52 weeks of age with 0.5 ml dose of 104.0 EID50 virulent ND field virus. All the vaccinated groups in this study showed protective level of antibody titre throughout the study period of 52 weeks. The challenged birds were observed for ten days for the development of clinical symptoms, lesions and mortality. No mortality was observed in any of the vaccinated group whereas 100 percent mortality was recorded during the observation period in the unvaccinated control groups.

Keywords

Oral pellet vaccines, desi chicken, ELISA test, immune response.

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