Niranjan Prakashrao Patil1*, Avinash D. Bholay2, Balu P. Kapadnis3 and Vishwas B. Gaikwad2
1Department of Microbiology, Abasaheb Garware College, Pune, Maharashtra, India. 2Department of Environmental Sciences, KTHM College, Nashik, Maharashtra, India. 3Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra, India.
Received on 17 June 2016 and accepted on 20 August 2016
The synthetic dyes are complex and resist microbial degradation, posing serious threat to the environment. The present study investigate methyl red (MR) decolorization and degradation potential of Bacillus circulans NPP1 isolated and identified from textile effluent treatment plant. The Bacillus circulans NPP1 decolorized greater than 98% of 50 ppm MR under optimal conditions (static, 4.5 hr, pH 7.5, 35oC temperature and 1% inoculum size of OD600=0.1).The isolate exhibited efficient decolorization of broad spectrum of dyes (nine dyes other than MR). The combination of glucose and yeast extract (YE) cosubstrates were found to be best for decolorization. The comparative analysis of decolorized broth and control confirmed azoreduction of MR into component metabolites by employing UV/vis spectrometry, FTIR and GCMS techniques. The induction of various oxidative (lignin peroxidases, laccases and tyrosinases) and reductive enzyme (azoreductase) indicated their involvement in decolorization and biodegradation process. The results of phytotoxicity test on seeds of Sorghum bicolor and Pennisteum americanum, revealed less toxic nature of degraded product as compare to parent MR dye. This study emphasizes the potential of Bacillus circulans NPP1 for bioremediation of dye contaminated effluent.
Methyl red; Bacillus circulans; Biodegradation; Textile effluent; Decolorization.