ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Prachi Singh and Yogendra Singh
Department of Plant Pathology, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar – 263 145, U.S. Nagar, Uttarakhand, India.
J Pure Appl Microbiol. 2016;10(4):2747-2752
https://doi.org/10.22207/JPAM.10.4.33 | © The Author(s). 2016
Received: 11/05/2016 | Accepted: 28/07/2016 | Published: 31/12/2016
Abstract

Stalk rot caused by Erwinia chrysanthemi (Ech) is one of the most destructive diseases of sorghum crop. The bacterium was isolated from infected sorghum plants collected from livestock research centre, G.B. Pant University of Agriculture and Technology, Pantnagar, India. Evaluation of inoculation methods viz. leaf whorl inoculation, stem injection method, root tip cut dip method and tooth pick method was done in glasshouse by inoculation from 24 h old culture of Ech adjusted to 1×106  cfu/ml, 1×107 cfu/ml and 1×108 cfu/ml by adding sterilized distilled water and 0.7% (v/v) of tween-40 (surfactant). Leaf whorl inoculation, stem injection and root tip cut and dip method which showed significant results in glass house were further used for field experimentation. Root tip cut and dip method was observed to be the best inoculation method both in glasshouse and field experimentation and the optimum infection was reported at 1×107 cells/ml (cfu).

Keywords

Stalk rot, Erwinia chrysanthemi, leaf whorl inoculation, stem injection method, root tip cut dip method, tooth pick method.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.