Roya Torabizadeh1,2*,Gita Eslami2, Mohammad Hossein Dehghantarzejani3, Zahra Zahirnia2 and Malihe Habibi2

1Departement of Medical Microbiology, Alborz university of Medical Sciences, Karaj, Iran.
2Departement of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3Departement of Medical Biochemistry, Alborz University of Medical Sciences, Karaj, Iran.

ABSTRACT

Sexually transmitted disease (STD) consist of Neisseria gonoroheae ,Chlamydia trachomatis ,Syphilis and Trichomonyasis are a major public health concern.The prevalence of these organisms are increasing level because of homosexuality ,migration and resistance to antibacterial agents and also usage of high technique for diagnosis of infection.according to WHO information ,88 million people from 448 million which infected with STD , is Neisseria gonoroheae ,wherase Iran as a developing country have not complete prevalence information about Neisseria gonoroheae , in this study it has been tried to estimate the prevalence of Neisseria. Among iranian women with phenotypin and genotyping methods. 300 specimens were enrolled in this study which collected from women with genital problems Dacron swab samples were cultured in chocolate agar in 37°C with 5-10% CO2 for 24-48hours. Gram staining, oxidase and carbohydrate utilization tests were used to identify the isolated species. Extraction of DNA of All isolates recognize as Neisseria gonoroheae were done , PCR for amplification of 390 bp fragment of CPPB plasmid for confirmation of isolates were performed. From 300 specimens , 7 isolates recognize as Neisseria gonoroheae with culture and PCR methods. It means N. gonorrhoeae was detected in 2.6% cases which enrolled in this study. The study revealed that although primary report found no prevalence of gonorrohea in iran. but other study revealed different prevalence of bacteria in IRAN ,even in the recent studies , the results are very near to this result .with consideration that gonoccocal infection have differrent sequelle in young age or pregnant women so it is very importat that with culture or PCR methods, screening of bacteria should be done.

Keywords:  Phenotyping and Genotyping methods, STD.