ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Ikram Medjaoui1 , Bouabdellah Rahmani2, Malika Talhi1, Fatima Zohra Mahammi1, Fatima Zohra Moghtit1, Nadhira Mehtar1 and Semir Bechir Suheil Gaouar1,3
1Laboratoire de Genetique Moleculaire et Cellulaire, Departement de Genetique Moleculaire Appliquée, Faculte des sciences de la nature et de la vie, Universite des Sciences et de la Technologie d’Oran- Mohamed BOUDIAF-USTOMB-, BP 1505 El M’naouer 31036, Oran, Algeria.
2Departement d’Electronique, Faculte de Genie  Electrique, Université d’Oran, BP 1524. El M’Naouar, 31000 Oran, Algeria.
3Departement de biologie, Universite Abou Bekr Belkaid, Tlemcen, Algeria.
J Pure Appl Microbiol. 2016;10(4):2613-2620
https://doi.org/10.22207/JPAM.10.4.17 | © The Author(s). 2016
Received: 08/10/2016 | Accepted: 24/11/2016 | Published: 31/12/2016
Abstract

Breast milk is a rich source of nutrients. It helps to establish a good intestinal flora of infants. In this context, we have isolated and characterized lactic acid bacteria from human milk and infant feces of the northwest Algerian population (Oran). In the present study, 87 bacterial strains were isolated from breast milk and infant feces samples from 20 mother-infant pairs. Isolates were identified by 16S rDNA sequencing analysis. The results of 16S rDNA sequencing analysis showed that the strains isolated from milk and faecal samples are part of 12 different species belonging to the genera: Enterococcus, Staphylococcus, Streptococcus and Lactobacillus. A homogeneous distribution was found for the four genera in the two media. Also, several mother/infant pairs shared some bacterial species belonging to different bacterial genera. Comparing the intestinal flora of vaginally delivered infants and those born by Caesarean section, a significant difference was found for the genus Staphylococcus (P-value <0.05) with a high rate among infants born by cesarean section. These results indicate that breast milk and delivery mode may contribute to the infant gut colonization.

Keywords

Lactic acid bacteria; human milk; infant feces; 16S rDNA sequencing analysis; vaginally delivery; cesarean section.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.