Manoj Kumar Maurya, Mukesh Srivastva, Sonika Pandey, Mohd.Shahid and Ved Ratan

Department of Plant Pathology, C.S. Azad University of Agriculture & Technology, Kanpur, U.P. 208002 India.

Received on 13 June 2016 and accepted on 19 September 2016

 

ABSTRACT

In vitro effect on chickpea seedling induced by biopriming of Trichoderma viride ie. the treatment T1(T. viride 5g/kg seed) was excelled overall significant superior performance by contributing 68%, 12.07cm, 16.37cm, 28.44cm, 0.592g, 1933.92 and 40.26 in germination, root length, shoot length, seedling length, dry weight, vigour index I and vigour index II, respectively. The highest protein content was observed in chickpea seedling that’s bioprimed by Trichoderma viride formulation (T. viride @5g/kg seeds) was estimated through Kelplus analizer was 0.41%.The protein quantification of chickpea seedlings was estimated through Lowry and Bradford method were 1.06 and 1.08 mg /ml. The protein profiling of chickpea seedling on SDS PAGE were observed maximum five bands, first band at 250 kda, second band at 100 kda, third band was lying between 75 to50 kda, and fourth band was at 20 kda.

Keywords

Chickpea seedling, biopriming, Trichoderma viride and vigour index.