ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Nadjia Benhamed1,2 , Gautier Philippe3, Mezouar Ismahene1, Donnio Pièrre Yves3 and Kihal Mebrouk2
1Department of Applied Molecular Genetics, Faculty of Nature and Life Sciences, University of Sciences Technologies of Oran –Mohammed Boudiaf (USTO-MB), BP 1505 El M’naouar Bir Eldjir Oran 31000, Oran, Algeria.
2Laboratory of Applied Microbiology, Department of Biology, Faculty of Nature and Life Sciences,  University Oran 1, Ahmed Ben Bella, BP 16, ES-senia 31000,Oran, Algeria.
3Unit of Microbiology, University of   Rennes 1, 35043, Rennes, France.
J Pure Appl Microbiol. 2016;10(4):2557-2562 | © The Author(s). 2016
Received: 02/10/2016 | Accepted: 19/11/2016 | Published: 31/12/2016

Staphylococcus aureus causes one of the most common types of chronic mastitis. Mastitis is an inflammation of the mammary gland with local and symptoms that occasionally result in a systemic infection. This disease has profound impact on animal welfare and milk quality. This study allowed us to determine the MLST and spa typing diversity of Staphylococcus aureus isolated from cases of bovine mastitis in the west Algeria. The first purpose of this work was to investigate the phylogenetic profile of Staphylococcus aureus by spa typing and Multilocus sequence typing (MLST) isolated from Mastitis subcliniques and clinical cases which were previously investigated by Mass Spectrometry Maldi-Tof and spa typing. The second purpose was to determinate the complex clonal of strains. In total, 213 quarters milk samples were collected from 56 dairy cows at 6 farms in the region of Oran, from 2011 to 2012. The quarter milk samples were bacteriological analyzed and scored using California Mastitis Test. The prevalence-isolated bacteria were Staphylococci (38.9%)


Mastitis, Staphylococcus aureus, identification, MLST, spa, phylogeny.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.