ISSN: 0973-7510

E-ISSN: 2581-690X

S. Senthil Kumar , T. Shariq Afsar, M. Mohamed Yasar, A. Mansoor Hussain and M.S. Mohamed Jaabir
Department of Biotechnology, Jamal Mohamed College (Autonomous), Tiruchirappalli – 620 020, India.
J Pure Appl Microbiol. 2010;4(1):429-432
© The Author(s). 2010
Received: 26/11/2009 | Accepted: 21/04/2010| Published: 30/04/2010

Biological control of plant pathogens by soil-bacteria is a well established phenomenon and Chitinase production has been shown to play a role in the suppression of various diseases. The interest in chitin degrading enzymes and their application in management of fungal pathogens are significant because chitin is an important constituent of the cell walls of filamentous fungi, composed of b-1,4 linked units of the amino sugar N-acetyl-D-glucosamine (NAGA), speculated to play a vital role in fungal defense against harm and pressures. Chitinolytic bacterial strains were isolated from prawn culture farms of Ramanathapuram District, Tamil Nadu. Soil samples was serially diluted and inoculated on colloidal chitin agar (CCA) medium. Strains exhibiting clear zones around colony were picked and further subjected to antifungal activity by hyphal extension inhibition assay against Rhizactonia solani, Trichoderma viride, Alternaria solani, Sclerotium rolfsii, Fusarium sp., Colletotrichum sp. and Aspergillus sp. The potential Chitinase producing organism was identified to be Bacillus subtilis JMC02 by 16SrRNA gene partial sequence.


Chitinase, chitinolytic bacteria, hyphal inihibition assay, fungal antagonism, Bacillus subtilis JMC02

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