ISSN: 0973-7510

E-ISSN: 2581-690X

Bharat Singh, Vijayalatha Venugopalan, Rakha H. Das, and Hemant K. Gautam
Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi-110 007, India
J Pure Appl Microbiol. 2008;2(1):205-210
© The Author(s). 2008
Received: 06/03/2008 | Accepted: 16/04/2008 | Published: 30/04/2008

A simple, cost effective and rapid method is described for the separation of total community DNA and humic substances from the soil samples for metagenomic studies.
This method was based on hot detergent lyses method for isolation of less sheared high molecular DNA from any soil sample followed by gel exclusion chromatography for complete removal of coextracted humic substances. Purity of the DNA samples isolated by this method was confirmed by restriction digestion and by the amplification of 16S rRNA gene. Four highly contaminated soil samples were evaluated by this method and recovery was found to be
12-20 µg/g of soil with average size > 25kb in 6-7 hrs.  This method can be used as a substitute for expensive kits used in rapid separation of DNA and humic substances from the  soil samples.


Humic Acid, Metagenomic DNA, 16SrRNA, Restriction and Digestions

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© The Author(s) 2008. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.