ISSN: 0973-7510

E-ISSN: 2581-690X

Taha A. Tewfike1 , Hanaa H.A. Gomaa2 and Entsar A. Nassar3
1Botany Department (Microbiology), Faculty of Agriculture, Benha University, Egypt.
2Botany Department, Faculty of Science, Suez canal University, Egypt.
3Botany and Microbiology Department, Faculty of Science, Helwan University, Egypt.
J. Pure Appl. Microbiol., 2016, 10 (1): 129-137
© The Author(s). 2016
Received: 02/10/2015 | Accepted: 16/12/2015 | Published: 31/03/2016
Abstract

This study aims to apply a novel strategy to explore the possibilities of utilizing rubber nanocomposite borne bacteriophages for removal of pathogenic bacteria in hospital wastewater. Samples of wastewater were collected from different hospitals in Benha city, Egypt. The collected samples had acceptable pH level (within WHO standards) but high chemical oxygen demand (COD) and very low of dissolved oxygen (DO) levels (out WHO standard). The treated wastewater with  nanocomposite or/and phages had physicochemical characters within WHO standard; as well as reduced total viable and spore forming bacteria(CFU); total coliform (TC); fecal coliform (FC) and fecal Streptococci (FS) population. They were reduced from 12×10 6 ; 15×103 ; 13X105 ; 12X103 and 2.5×102 to zero CFU/ml-1  for water sample respectively. The isolated bacteria Escherichia spp.; Pseudomonas spp., Salmonella spp. and Staphylococcus spp. were reduced to 110, 80, 50 and 30 CFU/ml-1 of wastewater respectively. Escherichia sp. has the highest frequency of antibiotic resistance followed by Pseudomonas sp., Salmonella sp. and Staphylococcus sp. The specific lysate phages against isolated pathogen bacteria were isolated from hospital waste water. Phages treatments have the potential to eliminate isolated bacteria. They have been standardized as 20X101, 5.2X101, 3X101and 2.2 X101 PFU/ml-1 respectively. The application of nanocomposite borne specific phage lysate resulted in 100% removal of pathogens from hospital wastewater after 20 hours of phage treatment.

Keywords

Multidrug bacteria, Pathogenic bacteria, Phages, Rubber nanocomposite, Wastewater.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.