ISSN: 0973-7510

E-ISSN: 2581-690X

C. Bala Sekharan1 , C. Rambabu2, B. Praveen kumar3 and D. Ravi Shankar4
1Department of Biotechnology, P.B.Siddhartha College of Arts & Science, Vijayawada – 520 010.
2Department of Biochemistry, Acharya Nagarjuna University, Post Graduate center, Nuzvid-521 201.
3Department of Biochemistry, P.B.Siddhartha College of Arts & Science, Vijayawada – 520 010.
4Department of Biochemistry, Vananchal Dental College, Jarkhand, India.
J Pure Appl Microbiol. 2008;2(1):257-259
© The Author(s). 2008
Received: 08/02/2008 | Accepted: 16/03/2008 | Published: 30/04/2008
Abstract

A simple, sensitive and reproducible spectrophotometric method is developed for the determination of propyl gallic acid. This method is based on oxidative coupling reaction between propyl gallic acid with orcinol in the presence of Hydrogen peroxide and enzyme horseradish peroxidase to produce colored chromogen (lmax at 550 nm).  Results of analysis were validated statistically and by recovery studies. This method is successfully employed for the determination of propyl gallic acid in oils.

Keywords

Propyl gallic acid, orcinol, Visible Spectrophotomety, Beer’s Law

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