A total of 20 human hydatid cysts fluid samples were collected during January, 2015 to July, 2015 from patients attended to Al- Karamah teaching hospital at Wasit Province. Genetic characterization of all isolates of Echinococcus granulosus from hydatid cyst was performed by sequencing analysis of fragment of mitochondrial (CO1) cytochrome C oxidase subunit 1 and NADH dehydrogenase subunit1 (ND1) genes to determine genotyping of E.granulosus in hyper-endemic areas of Wasit province.This work done by randomly choosing of five samples.The results of DNA sequencing showed genotype 1(G1)and genotype 3 (G3) and the sheep strain G1was more prevalent than buffalo strain G3.
Hydatid, Human, DNA sequence, Diagnosis.
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