ISSN: 0973-7510

E-ISSN: 2581-690X

Abdulsadah A. Rahi1 and Magda A. Ali2
1Department of Biology, College of Science, Wasit University, Kut, Iraq.
2Department of Microbiology, College of Medicine, Wasit University, Kut, Iraq.
J. Pure Appl. Microbiol., 2016, 10 (2): 1015-1020
© The Author(s). 2016
Received: 25/03/2016 | Accepted: 09/05/2016 | Published: 30/06/2016
Abstract

A total of 20 human hydatid cysts fluid samples were collected during January, 2015 to July, 2015 from patients attended to Al- Karamah teaching hospital at Wasit Province. Genetic characterization of all isolates of Echinococcus granulosus from hydatid cyst was performed by sequencing analysis of fragment of mitochondrial (CO1) cytochrome C oxidase subunit 1 and NADH dehydrogenase subunit1 (ND1) genes to determine genotyping of E.granulosus in hyper-endemic areas of Wasit province.This work done by randomly choosing of five samples.The results of DNA sequencing showed genotype 1(G1)and genotype 3 (G3) and the sheep strain G1was more prevalent than buffalo strain G3.

Keywords

Hydatid, Human, DNA sequence, Diagnosis.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.