ISSN: 0973-7510
E-ISSN: 2581-690X
Corynebacterium glutamicum mutant strain with higher yield of trehalose was obtained using UV-chemical mutagenesis. The yield increased to 284.4% compared to wild strain and then studied the enzymatic properties of five synthases for trehalose. The optimum pH value for trehalose synthase, MTSase, MTHase, TPS and TPP was 7.0, 7.5, 6.5, 6.5, 7.5 and the range of pH 6.5-8.0, pH 6.5-7.5, pH 6.0-7.5, pH 6.0-7.5, pH 6.5-8 with over 80% of highest activity, respectively. The optimum reaction temperature for them was 35°C, 35°C, 40°C, 35°C, 35°C with maintaining higher reactivity at the range of 30-35°C, 35-40°C, 30-45°C, 25-40°C, 30-35°C, respectively.
Trehalose, mutagenesis, enzymatic property
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