Stalk rot caused by Erwinia chrysanthemi (Ech) is one of the most destructive diseases of sorghum crop. The bacterium was isolated from infected sorghum plants collected from livestock research centre, G.B. Pant University of Agriculture and Technology, Pantnagar, India. Evaluation of inoculation methods viz. leaf whorl inoculation, stem injection method, root tip cut dip method and tooth pick method was done in glasshouse by inoculation from 24 h old culture of Ech adjusted to 1×10⁶  cfu/ml, 1×10⁷ cfu/ml and 1×10⁸ cfu/ml by adding sterilized distilled water and 0.7% (v/v) of tween-40 (surfactant). Leaf whorl inoculation, stem injection and root tip cut and dip method which showed significant results in glass house were further used for field experimentation. Root tip cut and dip method was observed to be the best inoculation method both in glasshouse and field experimentation and the optimum infection was reported at 1×10⁷ cells/ml (cfu).