Neisseria gonorrhoeae (NG) is a sexually transmitted pathogen with increasing treatment challenges due to the rise in antimicrobial resistance. The emergence of drug-resistant strains necessitates the need for alternative and novel strategies for treatment. The aim of this research work was to assess the antimicrobial, bactericidal, and biofilm inhibitory potential of Nagarmotha, Basil, Sugandhmantri, Palmarosa, Eucalyptus, Lemongrass, Spearmint, Ginger, and Peppermint essential oils (EOs) against MDR clinical isolates of NG. The study was approved by the Institutional Ethics Committee, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, India. Endocervical/vaginal swab samples (n = 223) were collected from women aged 20-55 years, presenting with one or more symptoms of sexually transmitted infections (STIs). Clinical samples were cultured on specific media for the identification of NG. AST of standard antibiotics was performed using the Kirby-Bauer disc diffusion method. The antibacterial effect of selected EOs was assessed using agar well diffusion, and MIC and MBC were determined by broth microdilution methods. Biofilm inhibition was evaluated using a microtiter plate assay. Out of 223 swab samples, 100 (44.84%) were tested positive for NG. AST revealed that 36 isolates were MDR. Nagarmotha and Sugandhmantri essential oils exhibited the lowest MIC (20.83 µg/ml) and MBC (83.83 µg/ml) values for both. Nagarmotha and Sugandhmantri essential oils also showed high biofilm inhibition by 50.87% and 43.85% at 1/2 MIC concentration, respectively. GC-MS analysis indicated presence of Cyprene (45.64%) in Nargarmotha and Linalool (40.19%) in Sugandhmantri, as the major compounds. The findings suggest that Sugandhmantri and Nagarmotha essential oils, or their major phytochemicals may have promising applications in management of NG infections.