Agriculture plays a crucial role in Indonesia’s economy, and milk is recognized as a vital component of the human diet due to its high nutritional value. In Indonesia, some farmers do not immediately transport their milk to Village Unit Cooperatives, which serve as Milk Collection Centres, leading to delays that can span several hours. Delays of this type may result in the raw milk becoming low quality and tend to generate a lower farmer payout price. The objective of this research was to determine the dynamics of milk quality immediately post-milking after 0, 2, and 4 h of storage. Samples from seven milk cans were collected in May 2025 at Lucky Farm, Medowo, Kediri Regency, East Java. The samples were further separated into two groups for TPC and Lactoscan analysis. Three treatments were tested: T0 (baseline) at 0 hours; T1, stored in an incubator at 28 °C for 2 hours; and T2, stored under the same conditions for 4 hours. Statistical analysis showed that TPC results differed significantly between treatments 4.93, 5.14 and 5.65 cfu/mL of milk for T0, T1 and T2, respectively. The Lactoscan analysis revealed significant changes in fat, density, and salt content. These data highlight the importance of delivering milk to collection centers as quickly as possible to minimize bacterial growth and maintain optimal quality.