The study was conducted to observe the bacteriological profile of diabetic foot ulcers in patients at tertiary care hospital. The incidence and pattern of antibiotic resistance of MRSA was studied. A cross-sectional study was carried out in the tertiary care hospital from September 2017 to December 2021. Of a total of 313 DFU cases analysed, 304 cases were infected ulcers accounting for 97.13% of cases and the remaining 09 cases (2.87%) were noninfected. Of the isolates cultured from the infected ulcers, 261 (53.81%) were gram-negative organisms and 224 (46.18%) were gram-positive. Of the gram-positive organisms, Methicillin resistant Staphylococcus aureus (66.9%) was most common, followed by Methicillin sensitive Staphylococcus aureus (31.3%) and Streptococcus pyogenes (1.8%). Of the gram-negative organisms, Pseudomonas aeruginosa 67 (25.7%) was predominant, followed by Escherichia coli 53 (20.3%), Klebsiella species (19.2%), Acinetobacter species 50 (19.2%). The drug resistance pattern of MRSA was, Ofloxacin 82.6%, ciprofloxacin (89.3%), levofloxacin (91.3%), cefoxitin (100%) with Benzyl penicillin showing highest resistance of 100%. There was a significant rise of MRSA infections in DFUs.