A sustainable and environmentally beneficial method is the synthesis of green nanoparticles using various plant components. Extracts from medicinal and herbal plants were used to synthesize nano silver. Aqueous extracts of Solenostemma argel, Citrullus colocynthis, Syzygium aromaticum, Foeniculum vulgare, Maerua crassifolia, and Elettaria cardamomum have been tested as antimicrobial and antifungal agents. In a lab-scale system, the effects of these plant extracts with or without silver nanoparticles (AgNPs) were tested and evaluated. The plant extracts used were Solenostemma argel (SA-AgNPs), Citrullus colocynthis (CC-AgNPs), Syzygium aromaticum (SAR-AgNPs), Foeniculum vulgare (FV-AgNPs), Maerua crassifolia (MC-AgNPs), and Elettaria cardamomum (EC-AgNPs). According to the findings of the antibacterial tests, SA-AgNPs was the most effective plant extract combined with AgNPs, demonstrating high antibacterial activity. Conversely, the extracts from Solenostemma argel resulted in the most significant inhibitions of Candida albicans fungus growth. The potential bioassay activity of these synthetic nanoparticles were tested alongside the identical plant extracts (without the silver nanoparticles). Solenostemma argel exhibited the maximum zone of inhibition towards Bacillus subtilis (35 mm) and Candida albicans (34 mm), indicating its strong antimicrobial properties. Antibacterial activity results indicate that plant extracts combined with AgNPs possess promising antimicrobial activities against a range of pathogenic microorganisms. This study is crucial to the progression of green chemistry and may result in the development of novel antibacterial and antifungal agents that capitalize on the natural properties of medicinal plants while eliminating the need for toxic chemicals. This study recommends using medicinal plant extracts enhanced with AgNPs to control human pathogenic microbes, including Gram-positive, Gram-negative bacteria and yeasts.