ISSN: 0973-7510
E-ISSN: 2581-690X
The lead nitrate removal by isolated lead tolerant bacteria Pseudomonas sp isolated from electronic waste (e-waste) recycling facility soil was studied in a laboratory simulated environment. The bacterium was identified by biochemical and 16S rRNA molecular studies. The DNA was isolated and amplified using universal bacterial primers. The phylogeny tree suggested the isolated lead tolerant bacterium as Pseudomonas aeruginosa. The partial 16S rRNA sequences were submitted to NCBI Genbank and the accession number JN102340 was assigned to the submitted sequences. Various parameters were optimized for lead nitrate removal such as pH, temperature, kinetics studies and metal tolerant assay. The metal removal studies suggested that the lead tolerant Pseudomonas aeruginosa JN102340 able to remove lead nitrate 92% at pH 6, 89% at 35ºC, 90% after 24h incubation in the optimized pH and temperature. The bacterium Pseudomonas aeruginosa JN102340 tolerate and survive up to 600mg L-1 lead nitrate.
Bioremediation, Lead removal, Pseudomonas aeruginosa, Bacteria, e-waste, 16S rRNA
© The Author(s) 2013. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.