As an obligate intracellular, Gram-negative microorganism, Chlamydia trachomatis represents a prevalent sexually transmitted pathogen. It disproportionately impacts women of reproductive age, resulting in a spectrum of reproductive health complications. Despite the significant public health implications of this infection, its epidemiological characteristics in India remain inadequately documented, with a limited number of studies conducted. Endocervical swabs and serum samples were collected from 75 participants. A solid-phase immunochromatographic assay was employed for the detection of antigens within the endocervical specimens. In accordance with the manufacturer’s protocols, an enzyme immunoassay was utilized to ascertain the presence of IgM, IgG, and IgA antibodies in the serum specimens. For the purposes of this investigation, we recruited 75 patients exhibiting various symptoms indicative of lower genital tract infections. Among the symptomatic cohort, thirteen cases tested positive for chlamydia antibodies. Notably, two of these thirteen patients exhibited the presence of both IgG and IgA antibodies. Chlamydia antibodies were identified in 17.3% (13/75) of the symptomatic patients, as determined by the results of the antibody detection assays. In relation to patients diagnosed with cervicitis, 61.5% tested positive and 19.4% tested negative for chlamydia antibodies (P value = 0.0040).