Biofilms are surface-attached bacterial communities that are widespread in nature. A key factor in their development is quorum sensing (QS), a mechanism of intercellular communication among bacteria. Biofilm formation serves as a pivotal mechanism in the establishment of multidrug-resistance, significantly undermining the efficacy of conventional treatment strategies. The development of novel antimicrobial compounds that can disrupt or prevent biofilm formation has emerged as a critical strategy to mitigate the clinical impact of biofilm-associated infections. Medical devices are also receiving much attention in the healthcare sector to address this issue. Emerging studies suggests that both herbal formulations and synthetic agents are extensively explored for their prospective anti-biofilm efficacy. This review summarizes the mechanisms behind biofilm formation of commonly reported bacterial pathogens and regular techniques in medical device fabrication to control biofilm formation. Additionally, it focuses on the information about reported natural compounds as quorum quenching agents. Through an integrative analysis of biofilm mechanisms, anti-biofilm agents, and device fabrication techniques, this review offers critical perspectives for the development of clinically viable multi-targeted interventions for managing biofilm-driven medical complications.