The vaginal microbiome plays a vital role in maintaining reproductive health. Vaginal pH serves as a simple yet reliable clinical marker reflecting microbial balance. A healthy vaginal environment is typically dominated by Lactobacillus species, which help maintain an acidic pH that inhibits colonization by pathogenic organisms. An increase in vaginal pH disrupts this balance and is associated with leukorrhea, infertility, preterm birth, and increased susceptibility to sexually transmitted infections (STIs). This study aimed to determine the association between vaginal pH and microbial community composition in women attending a tertiary healthcare center. A total of 50 vaginal swab samples were analysed using standard culture and biochemical methods, and vaginal pH was categorized as low (≤4.5), mid (4.6-5.5), or high (>5.5). Elevated pH values were frequently associated with pathogenic organisms such as Escherichia coli, Klebsiella pneumoniae, and Gardnerella vaginalis, whereas low pH corresponded to predominance of Lactobacillus species. Candida albicans was detected across a wide pH range. These findings demonstrate that vaginal pH is closely linked to microbial profiles, where high pH reflects dysbiosis and low pH indicates a healthy vaginal ecosystem. Routine monitoring of vaginal pH may therefore serve as a useful tool in guiding diagnostic and therapeutic decisions in gynecological practice.