The goal of the present investigation was to demonstrate the antibacterial activity of different solvent extracts (methanol, ethanol, cold aqueous and hot aqueous) of Crassocephalum crepidioides against ATCC bacterial cultures of Staphylococcus aureus, Escherichia coli, Methicillin resistant Staphylococcus aureus, Pseudomonas aeruginosa and its antioxidant potential. Furthermore, the chemical constituents present in the extract was perused by Gas chromatography-mass spectroscopy (GC-MS), along with in vitro cytotoxicity assessment. All the extracts were shown to be sensitive against S. aureus, MRSA and P. aeruginosa except for the ethanolic extract which was resistant to P. aeruginosa. Of all the extracts, hot aqueous extract found to be the most effective. It was found that Minimum inhibitory concentration (MIC) value of hot aqueous extract against S. aureus, MRSA and P. aeruginosa were 5 mg/mL, 5 mg/mL and 40 mg/mL, respectively. DPPH results showed that C. crepidioides leaf extract has potent antioxidant activity with IC50 value of 57.9 µg/mL. 22 compounds were detected in hot aqueous extract through Gas chromatography-mass spectroscopy. The results of the cytotoxicity evaluation displayed that the IC50 value of the hot aqueous extract of C. crepidioides on Vero cell lines was 292 µg/mL. This study concludes that C. crepidioides leaf extract is non-toxic, has various bioactive components and strong antibacterial and antioxidant activities, thus making it a promising therapeutic agent for various biomedical applications.