0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.17.2.44 Dengue Virus Serotypes Circulating among Aedes Mosquitoes in the Lucknow District of North India: Molecular Identification and Characterization Srivastava Nikky Nyari Maan Harjeet Singh Dhole T.N. Singh Jasmeet Sharma Swati Pandey Shyam Narain Agarwal Ankita Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India. State Virology Laboratory, Department of Microbiology, Gandhi Medical College, Bhopal, Madhya Pradesh, India. Department of Botany, Lucknow University, Uttar Pradesh, India. 30 05 2023 17 2 1141 1153 Copyright © 2023 The Author(s) 2023 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Dengue is one of the most critical diseases, caused by Dengue virus (DENV) serotypes (DENV-1 to DENV-4). Study aims to detect DENV in natural A. aegypti from endemic regions of Uttar Pradesh. From 2010- 2013, mosquitoes collected from Uttar Pradesh tested for Dengue virus serotypes by capsid-premembrane gene-based PCR analyzed for genotypes sequencing of the C-prM junction of DENV genome. A total of 4731, 53.54% (n=2671) A. aegypti and 46 % (n=2060) A. albopictus mosquitoes were collected. Of 226 mosquito pools, 10 pools of A. aegypti and 14 pools of A. albopictus were positive for DENV by PCR. All 24 isolates identified as DENV-I; Genotype (G)-III (n=8), G-V (n=1); DENV-2; G-IV (n=4); DENV-3; G-III (n=11). The overall minimum infection rate was much higher in A. albopictus mosquitoes and presence of MIR in male mosquitoes is an indicating natural vertical transmission and important observation in geographical area indicating natural vertical transmission.

 Keywords Aedes Mosquitoes Entomological Surveillance Dengue Virus Vertical Transmission Polymerase Chain Reaction