0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.17.1.11 In vitro Antibacterial Potency of Leaf Extract of Moringa oleifera against NFGNB Isolated from UTI Patients and their Plasmid Profiling Shriwastav Shalini Kaur Narinder Bala Rosy Dev Jai Hassan Mahmudul Harshita Khatana Chainika Mittal Divya Aman Shahbaz Chauhan Jyoti Department of Microbiology, MMIMSR, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, India. Department of Microbiology, AIMSR, Adesh University, Bhatinda, Punjab, India. Department of Biotechnology, MMEC, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, India. Central Research Laboratory, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, India. Department of Microbiology, Shoolini University of Biotechnology and Management Sciences, Bajhol, Himachal Pradesh, India. 24 01 2023 17 1 222 230 Copyright © 2023 The Author(s) 2023 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Non-fermenting gram-negative bacteria (NFGNB) endeavouring as major pathogen in infectious disease, predominantly in urinary tract infection (UTI ) and increased resistance in NFGNB are matter of concern. This study aimed to evaluate the frequency of NFGNB, antibiotics resistance pattern, plasmid profiling, and antibacterial efficacy of Moringa oleifera against NFGNB. NFGNB were isolated from clinically suspected UTI patients. Identification of isolates and their antibiotics sensitivity pattern were analyzed according to conventional method, and Vitek 2 automated system. Moreover, NFGNB were evaluated for biofilm production and presence of plasmid. Furthermore, antibacterial activity of Moringa oleifera was evaluated against NFGNB. P. aeruginosa (86.0%), and A. baumannii (10.0%) were the most frequent NFGNB followed by Providencia rettgeri 2.0%, Stenotrophomonas maltophilia 1.0%, myroides species 1.0%. 68.6% P. aeruginosa and 60.0% A. baumannii were biofilm producers whereas imipenem and meropenem were the most effective antibiotics. Isolated NFGNB showed multiple bands of plasmid. Furthermore, Moringa oleifera leaves extract showed antibacterial activity against tested NFGNB. MDR-NFGNB presents challenges in treatment and Moringa oleifera leaf extract may be used as an alternative medicine. However, the therapeutic role of specific ingredients present in extract needs further investigation and purification.

 Keywords Multi-drug Resistant Uropathogen Non-fermenting Gram-negative Bacteria Plasmid Profile Analysis Moringa oleifera