0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.16.4.61 Antibacterial Effect of Silver Nanoparticles Prepared from Sophora flavescens Root Aqueous Extracts against Multidrug-resistance Pseudomonas aeruginosa and Staphylococcus aureus Al-Hayanni Huda S.A. Alnuaimi Marwah Th. AL-Lami Rana AH. Zaboon Shaimaa M. Department of Biology, College of Science for Women, University of Baghdad, Iraq. Ministry of Science & Technology, Directorate of Environment and Water, Baghdad, Iraq. Department of Biology, College of Science for women, University of Baghdad, Iraq. Ministry of Health, Iraq. 01 12 2022 16 4 2880 2890 Copyright © 2022 The Author(s) 2022 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Green synthesis of metal nanoparticles (NPs) from plant extracts has attracted significant interest in modern medicine. Therefore, this study prepared an aqueous extract of Sophora flavescens roots, which are used in folk medicine to treat several diseases, including bacterial infections. In addition, silver NPs (AgNPs) were synthesized from root extract using the green synthesis method. The NPs were diagnosed using modern methods. Additionally, the antibacterial activity of the root aqueous extract and AgNPs aqueous preparation (at concentrations of 7% and 9%, respectively) was examined against selected isolates of multidrug-resistant Pseudomonas aeruginosa and Staphylococcus aureus. The results indicated that both the plant extract and NP preparations inhibited pathogenic bacterial isolates.

Keywords Antibacterial Activity Green Synthesis Multidrug-resistance Bacteria Silver Nanoparticles Sophora flavescens