0973-7510 Journal of Pure and Applied Microbiology 2581-690X DR. M.N. Khan 10.22207/JPAM.16.1.55 Screening and Isolation of Bacteria Producing Biosurfactants from Waste Shagufta Shaikh M Dharani P.V. Department of Microbiology, Sheth P.T. Arts and Science College, Shri Govind Guru University, Godhra – 389 001, Gujarat, India. 25 02 2022 16 1 567 577 Copyright © 2022 The Author(s) 2022 This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.https://creativecommons.org/licenses/by/4.0/

Biosurfactant are surface active compounds (SAC), amphiphilic in nature consisting of hydrophobic (long chain fatty acids) and hydrophilic domain (amino acid, protein, carbohydrate). Classification of this compound is based on molecular weight, mode of action and physico-chemical structure. Biosurfactant are being utilized in laundry formulation, house hold cleaning products, as antimicrobial agents, in detergents, pesticides, food and pharmaceutical industries. Various type of microorganism has been studied capable of synthesizing wide range biosurfactant by utilizing hydrocarbon compounds as source of carbon and energy. Present study aims to isolate biosurfactant producing bacteria from different waste sources. In this study among twenty-one isolated microbial cultures from different waste samples were screened for biosurfactant production using methods like hemolytic activity, blue gar assay, hydrocarbon tolerance assay, oil spread test, Emulsification index (E24%).

Keywords Biosurfactant Oil Spreading Method Emulsification index Modified Drop Collapse Tolerance against Hydrocarbon Hemolytic activity Blue agar plate Agar well diffusion