Coloring agents such as allura red and tartrazine are frequently used in food and pharmaceutical industries. Also, many types of preservatives are added to food and pharmaceutical products to prevent their spoilage. This study examined the effect of pretreatment with nutrient broth containing allura red (AR) or tartrazine (TA) on survival of E. coli and Staphylococcus aureus in media containing preservatives (eg ascorbic acid, citric acid, or sodium chloride). Pretreatment of E. coli for 4 days with AR or TA before exposure for 3 days to nutrient broth containing NaCl (20%) significantly increased its resistance to sodium chloride (20%). Pretreatment of E. coli for 4 days with AR, TA, or nutrient broth alone before exposure for 3 days to nutrient broth containing ascorbic acid (1%) or to nutrient broth containing citric acid (0.5%) killed all cells. For S. aureus, pretreatment with AR or TA before exposure for 3 days to nutrient broth containing citric acid (0.3%) significantly increased its resistance to citric acid (0.3%). Pretreatment of S. aureus for 4 days with AR, TA, or nutrient broth alone before exposure for 3 days to nutrient broth containing citric acid (0.3%) reduced number of cells by 4.37, 2.54, and 6.97 logs, respectively. Pretreatment of S. aureus for 4 days with AR, TA, or nutrient broth alone before exposure for 3 days to nutrient broth containing ascorbic acid (0.5%) reduced number of viable cells by 3, 3.6, and 3.07 logs, respectively. Pretreatment of S. aureus for 4 days with AR, TA, or nutrient broth alone before exposure for 3 days to nutrient broth containing NaCl (20%) increased number of cells by 0.74, 0.7 and 1.1logs, respectively. These results have implications in food and pharmaceutical industries.