Urinary tract infection (UTI) is one of the most delicate health problem affecting women with severe complications and the rising antimicrobial resistance exerts a massive influence in treating UTIs. The objectives of this study was to identify the uropathogens causing UTI in non-pregnant women and their antibiotic resistance pattern. The study was undertaken in the outpatient department (OPD) of Obstetrics and Gynaecology, Capital Hospital, Bhubaneswar, Odisha during April 2015 to March 2016. A total of 766 midstream urine samples (MSU) was collected from women of different age. Out of 766 urine samples, 323 (42.1%) samples were culture positive for UTI with prominent bacteriuria. E.coli was found to be the most common isolate (54.79 %) followed by Staphylococcus sp. (22.6%), Enterobacter sp. (17.64%) and Klebsiella sp. (4.95%). Amongst various clinical symptoms, burning and itching during urination was found to be the most common (69.97%) symptoms followed by abnormal discharge of the vagina (45.20%) and the presence of cloudy urine (24.46%). E.coli the most predominant uropathogen identified was found to be highly susceptible towards a low level of resistance towards nitrofurantoin (11.8%) followed by amikacin (20.03%) and gentamicin (22.0%). Our study revealed that E. coli isolates were the predominant uropathogens and showed escalating model of resistance to the general available antimicrobial agents which are frequently used by the physicians in hospitals and private practice.