Acinetobacter baumannii became a serious endemic and widespread pathogen responsible for causing nosocomial infections due to restricted treatment options. This study was conducted to evaluate the role of both antibiotic resistance and genetic pattern in infection caused by MBLs resistance A. baumannii isolated in Baghdad hospitals. Finally, developing selective media in order to detect multidrug resistance A. baumannii isolates according to colony shape appearance changing. Collected 124 isolates from various clinical and environmental sources. Biofilm Formation was detected, Antibiotic sensitive for 21 antibiotic discs were determined, MDR index calculated, PCR was performed to investigate MBLs genes bla (IMP2, KPC,  DIM,  SPM, VIM, GIM, AIM, BIC,  SIM, NDM, Pre-NDM-A, GES, Imp-1, and VIM). Increasing resistant prevalence of A. baumannii appear significantly in higher rates to seven antibiotics group and MDR index ranged between 0.29 to 1.0 from 6-21 antibiotics. It was clear in Biofilm formation evaluated 75.8% strong biofilm after 72 hours, indicated that there is a significant negative correlation between biofilm formation capacity and the inherent ability of bacteria to show multidrug resist (P< 0.001). Our findings indicated that non-MDR isolates tended to form more robust biofilm formation. Metallo b-Lactamase detection phenotype showed 113/124 isolates able to produced ESBL, a result confirmed by PCR assay to detect the resistance of MBL-genes, appeared that a high percentage of bla_IMP 79% which left clinicians with limited treatment options. An increasing number of hospital MDR A. baumannii has been reported. Furthermore, the study will focus on the evolution and the emergence of multidrug resistance A. baumannii in Iraq. The common MBL gene is the bla_IMP