Rana A.Jawad, Ihsan M. Sulbi, Yasser J. Jameel and
Hayder A. Muhammid
College of Veterinary Medicine / University of Kerbala, Karbala, Iraq.
The aim of this study was to choose the optimal PCR technique with specific designing primer design of cystic fluid of Echinococcus granulosus hydatid cyst in cattle have slaughtered in kerbala abattoirs / kerbala governorate, the techniques have allowed to reduce the time consumption ,Nucleotide sequences of Echinococcus granulosus in Iraq cattle was obtained from NCBI websites with other researchers concerned in this topic, numerous visceral organs Livers , lungs and liver with lung suspected have hydatid cysts were isolated from 35 cow؛s slaughter, In PCR the highest number of positive results have 22(62.8%) were detected as Echinococcus granulosus hydatid cyst, on the other organs, the parasite isolation ratio was calculated from internal organs the percentage of hydatid cysts were significant increases as 12 (63.6%), 6 (27.2%)and 2(18.1%) for Liver, lung and liver with lung, respectively.
Keywords: Echinococcus granulosus ,hydatid cyst in cow and PCR.